H7790

Immunoblotting
Whole extract of human HEK-293T cells overexpressing human HRD1/SYVN1 was separated on SDS-PAGE and probed with Rabbit Anti-HRD1/SYVN1 (Cat. No. H7790). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 4 μg/mL antibody
2. 2 μg/mL antibody
3. 2 μg/mL antibody + HRD1/SYVN1 immunizing peptide (human, 195-211)

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H7790

Sigma

 

Anti-HRD1/SYVN1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym:Anti-Synovial apoptosis inhibitor 1, Anti-Synoviolin 1, Anti-Synoviolin

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Description

Immunogensynthetic peptide corresponding to amino acids 195-211 of human HRD1/SYVN1, conjugated to KLH. The corresponding sequence is identical in rat and mouse.
Physical formSolution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Properties

antibody formaffinity isolated antibody
clone(polyclonal)
formbuffered aqueous solution
mol wtantigen mol wt ~70 kDa
species reactivitymouse, human
concentration~1.0 mg/mL
application(s)immunoblotting: 2-4 μg/mL using whole extract of HEK-293T cells expressing human HRD1/SYVN1.
 immunoprecipitation: 5-10 μg using lysate of mouse brain
Gene Informationhuman ... SYVN1(84447)
 rat ... Syvn1(361712)
 mouse ... Syvn1(74126)
shipped indry ice
storage temp.−20°C

Safety

WGK Germany1